Product Description
3D StarPore® Max Microcarriers are independently developed and manufactured by Huachen Bio specifically for high-yield suspension culture of various animal cells—including mesenchymal stem cells, 293 cells, Vero cells, and more—with culture volumes ranging from a few milliliters to several hundred liters.Featuring excellent surface properties and a density slightly greater than water, these microcarriers are designed with a flexible porous structure made of gelatin, with particle sizes ranging from 200–350 μm. Each product specification is supplied with a defined weight, pre-sterilized and ready for immediate use.
Advantages of Microcarrier Culture
Increased Productivity: The microcarrier system offers an extremely high surface area-to-volume ratio, enabling high cell yields without the need for large-volume equipment or labor-intensive methods. Compared to other monolayer culture systems, microcarrier culture requires significantly less space to produce equivalent quantities of cells or cell-derived products.
Protection from Physical and Chemical Stress: Macroporous microcarriers shield cells from shear stress caused by impeller agitation, particularly during large-scale culture. This protective effect also allows for oxygenation using pure oxygen microbubbles.
Reduced Medium Consumption: Lower medium requirements translate into significant cost savings in cell culture processes.
Reduced Labor Requirements: By enabling high-density culture in smaller volumes, microcarrier systems reduce the need for large culture vessels. Cell harvesting is simplified: after stopping agitation, microcarriers with attached cells settle by gravity, allowing easy removal of the supernatant.
Lower Contamination Risk: Microcarrier culture minimizes the number of handling steps, substantially reducing the risk of contamination.
Product Features
SEM Image of Max Microcarriers
Morphology of Hydrated Microcarriers
Huachen 3D Large-Scale Culture Solutions
Single-Stage Process
Two-Stage Process
3D StarPore®Max Large Health Spinner Flask System
500mL Spinner Flask, One StarSpan™ Mini Bioreactor
4 Flask Positions, with a Maximum System Volume of 2L at a Time
One 500mL spinner flask can hold a 400mL culture system, with an inoculation density of 2g/L (total 0.8g) and 4×10⁷ cells inoculated. After 20-fold expansion, 8×10⁸ cells can be harvested from a single flask in 4 days, which is equivalent to one 10-layer cell factory. One mini bioreactor can harvest 3.2×10⁹ cells at a time, equivalent to four 10-layer cell factories.
Reduce incubator occupancy rate, labor input, and cell processing workload.
10L Bioreactor Cell Harvest Parameters
MSCs on 3D StarPore Microcarriers – Brightfield
MSCs on 3D StarPore Microcarriers – Fluorescence
20-Minute Complete Microcarrier Digestion
Day 4 Microcarrier Lysis & Harvest
Cell Counter Results
10L Bioreactor: Fold Expansion and Total Cell Yield
Metabolic Profile & Cell Size During Expansion
Quality Control
Flow Cytometry Analysis of Stem Cell Surface Markers
Quality Control
Endotoxin Testing in Cell Culture Supernatant
Endotoxin testing of Day 3 and Day 4 cell culture supernatants, filtered through a 0.22 µm membrane, showed results <0.1 EU/mL in all samples.
Results & Conclusions
Test Results
Cell Culture (2D Planar): Ten-layer Cell Factory culture showed normal growth, with a viability of 97.82%. Cell count met the requirements for bioreactor inoculation.
Cell Harvest (3D 10L Bioreactor): After 3 days of culture, cell count reached 9.983 billion, with a 16.64-fold expansion and 98.16% viability. After 4 days of culture, cells were washed with PBS, followed by microcarrier dissociation using lysis solution. A total of 12.18 billion cells were harvested, achieving a 20.3-fold expansion and 97.64% viability. The average cell diameter was 14.88 μm, meeting all acceptance criteria for harvest.
Cell Quality Control: Day 4 harvested cells were washed and cryopreserved. Residue testing for microcarriers and lysis solution in both the second wash and final cryopreservation formulation passed all specifications.
Cell Quality Control (Endotoxin): Endotoxin level in the Day 4 culture supernatant was <0.25 EU/mL, demonstrating an ultra-low endotoxin level throughout the entire culture system.
Conclusion
Cell Culture: Cell expansion fold (>20-fold by Day 4), cell count, and viability all met required specifications.
Cell Harvest: Cell viability (>95%) complied with acceptance criteria.
Cell Quality Control: Endotoxin levels and residue testing met all required specifications.
