3D StarPore gelatin dissolvable porous microcarrier for cell culture

货号：SPMax - Specifications

目录价：Inquiry

简介：

Gelatin Component with Flexible Porous Structure Design

Particle Size Ranging from 200 to 350 μm

All Raw Materials Are Sourced from GMP-Grade Production for Pharmaceutical Excipients

It has undergone irradiation sterilization and can be used directly

Suitable for Various Adherent Cells

Advantages of Microcarrier Culture

Enhancing Production Capacity: The microcarrier system provides an extremely high culture surface area-to-volume ratio, enabling high cell yields without relying on large-capacity equipment or cumbersome methods. Compared with other types of monolayer culture, microcarrier culture requires considerably less space to produce a certain quantity of cells or cell products.
Protecting Cells Against Physical and Chemical Stresses: Macroporous microcarriers can protect cells from the impact of stirrers, especially during large-scale culture. Thanks to this protective effect, it is also feasible to use pure oxygen microbubbles for sparging aeration.
Reducing Medium Requirements: The reduced demand for medium translates to significant savings in cell culture costs.
Lowering Labor Requirements: Since a large number of cells can be cultured in a small volume, microcarrier culture requires smaller culture vessels. Separating cells from the culture medium is straightforward: stop stirring, allow the microcarriers with adherent cells to settle by gravity, and then remove the supernatant.
Reducing the Risk of Contamination: Microcarrier culture decreases the number of handling steps, thereby greatly lowering the risk of contamination.

Eight Features of Huachen Bio 3D StarPore® Microcarriers

1、The 3D StarPore® Microcarrier is an independently developed product by Huachen Bio, specifically designed for high-yield suspension culture of various animal cells (such as mesenchymal stem cells, 293 cells, and Vero cells) with culture volumes ranging from several milliliters to hundreds of liters. It possesses excellent surface properties and optical properties, and has a specific gravity slightly higher than that of water

2、The flexible porous structure of the gelatin component allows cells to grow in the microcarriers, enabling natural coordination and binding with various cells, and facilitating easy cell attachment, growth, and harvesting

3、The microcarriers feature a continuously porous structure, which protects cells from shear stress damage and allows cells to create a microenvironment

4、With a particle size ranging from 200 to 350 μm and a rounded morphology, it is more suitable for the adhesion and expansion of adherent cells.

5、It allows for gentle digestion and can be completely dissolved in digestive solution. Digestion can be completed within 30 minutes, facilitating the harvesting of final cell preparations, with a viable cell recovery rate of up to 98%.

6、All raw materials are sourced from pharmaceutical excipients and produced in accordance with GMP standards

7、It is suitable for large-scale suspension culture systems of various adherent cells, such as mesenchymal stem cells, 293 cells, and Vero cells.

8、Its optimized surface characteristics are suitable for effective cell adhesion and spreading; the optimized size, density, and macroporosity facilitate uniform suspension and enable excellent growth and high yield of various cells.

Microsphere Material & Stem Cell Flow Chart

Adhesion and Proliferation of MSCs on Microcarriers

MSCs on microcarriers expand over time in a bioreactor: initially, MSCs attach with a low coverage rate in a rounded morphology, then flatten and spread,

followed by entering the growth phase and expanding to cover a large portion of the microcarrier surface area.