Brief Introduction to 
the Expansion Principle and Core Technology of hyperClone NK

HUACHEN hyperClone NK-kit integrates important signaling pathway factors essential for NK cell activation and expansion, as well as key cytokines that maintain and extend NK cell activity. Therefore, it can efficiently expand and activate high-purity NK cells, and serves as a powerful tool for large-scale preparation of NK cells with tumor-killing activity via the pure protein factor method.

hyperClone NK Cell Pure Protein Factor In Vitro Expansion Kit
It requires no feeder cells, enabling efficient expansion of NK cells with high purity to meet the application needs of autologous or allogeneic NK cells. With a defined chemical composition and compliance with GMP standards, it can support the regulatory filing of NK cell-based pharmaceuticals.

Sources of NK Cells

It is suitable for peripheral blood and umbilical cord blood, and compatible with both fresh blood 
samples and cryopreserved cells.

NK Cell Application and Operation Process

Data Presentation

Changes in Surface Marker Expression During PB-NK Cell Expansion

Example 2: Expansion Efficacy of CB-NK Cells

Detection of NK Cell Cytotoxicity

Tumor Killing Assay: Co-incubation of CBMCs with K562 Cells 
for 4 Hours After 14 Days of CBMC Culture

Tumor Killing Assay: Co-incubation of PBMCs with K562 Cells 
for 4 Hours After 14 Days of PBMC Culture

ADCC:RajiKilling ability Rituximab: 5 μg/mL, co-incubation for 4 hours

Data Presentation

Cytokine and Cytotoxic Mediator Secretion: CD107a

Cytokine and Cytotoxic Mediator Secretion: TNF-α, IFN-γ

Cytokine and Cytotoxic Mediator Secretion: Granzyme B

Cytokine and Cytotoxic Mediator Secretion: Perforin

Allogeneic NK Cell Application——
Screening and Matching for Cord Blood NK Cell Bank Establishment

Allogeneic NK Cell Therapy —— The Future of Cell Therapy Is Coming

The hyperClone NK Kit enables high-density, long-term culture and supports the acquisition of large quantities of

allogeneic NK cell ready-to-use formulations with high purity and high activity.

Innovative Application Strategy Based on HUACHEN-NK Cells
——Family NK Cell Healthcare

Family NK Cell Healthcare

A young member of the family donates a 50 mL blood sample

The laboratory performs expansion culture to establish 20–30 seed banks

Thawing one seed bank each time can support one NK cell healthcare session for a family member

Calculated based on two sessions per person per year, the seed banks can meet the family's needs for 15 years

Blood relatives who are young have cells with better activity, and these cells are more easily accepted (by the recipients' bodies).

Innovative Application Strategy Based on HUACHEN-NK Cells
——NK Cell Healthcare for High-Net-Worth Individuals

NK Cell Healthcare for High-Net-Worth Individuals

First, adjust the body to an optimal state, then collect 50 mL of blood、

Perform laboratory expansion and activation of the cells, followed by storage of 20–30 seed banks of cells

Retrieve one NK cell seed bank each time for two sessions annually, with two cell infusions per year

This setup can meet an individual’s needs for 15 years

These are autologous cells, activated in vitro with high purity. In the future, they can support cell-based gene-modified therapy.

Top 10 Product Advantages

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