HuaChen Bio hyperCryoCellTM Serum-free Cell Freezing Medium
The hyper-CryoCell Cryopreservation Media Series are pre-formulated, ready-to-use cryoprotectants designed for the preservation of stem cells and immune cell therapy products under ultra-low temperature conditions (-80°C and -196°C). All products in the series feature serum-free formulations and utilize clinical-grade injectable components. With superior formulation technology, they ensure safe and stable long-term cell storage with high post-thaw viability.
Application Cell Type
Stem cells: MSCs/HSC/iPSCs, etc.
Immune cells:PBMC/CAR-T/TCR-T/NK/CAR-NK/DC, etc.
Cell lines: NK92/CHO, etc.
Other sensitive cells and tissues requiring cryoprotection
DMF: 042976
hyperCryoCell™ Cryopreservation Media – Product List
Product Data
Instructions for Use
For optimal post-thaw viability, it is recommended to cryopreserve cells during the logarithmic growth phase.
01. Store hyperCryoCell medium at 4°C. Harvest cells, pass through a strainer to obtain a single-cell suspension, and perform cell counting.
02. Wash to remove culture medium. Aspirate the supernatant as thoroughly as possible to avoid diluting the cryopreservation medium.
03. Resuspend cells: Calculate the required volume of hyperCryoCell medium based on cell density (stem cells: 1E6–3E7/mL; immune cells: 1E7–2E8/mL). Add the appropriate volume of hyperCryoCell medium to the cell pellet, gently pipette to resuspend, and aliquot into cryovials or cryobags.
04. Mix by inversion, place in a controlled-rate freezing container, and immediately transfer to a -80°C freezer. Alternatively, use a programmable freezer for controlled nucleation before transferring to liquid nitrogen for long-term storage.
