High expansion fold: >100,000-fold expansion by Day 28; High cell quantity: approximately 200 billion cells by Day 21; High purity: >98% CD3⁻CD56⁺ (High-Efficiency Version), >90% CD3⁻CD56⁺ (Enhanced Version), and >90% CD16⁺CD56⁺ ; High cell viability: >90% viability; Low cost: The production cost per NK cell preparation is 1/3 to 1/5 of the original cost; Allogeneic use: CD3⁺ cells < 1%, meeting the requirement for allogeneic application; Pure factor: feeder-free, GMP-manufactured, with DMF filing number.
Special optimization for cryopreserved mononuclear cells; Suitable for cryopreserved cells of peripheral blood PBMCs and umbilical cord blood CBMCs; Starting with a cryopreserved cell count of 4–6×10⁷, using a T75 culture flask initially, and scaling up to a 2L culture system around day 14 of cultivation, 6–8×10⁹ NK cells can be obtained; The purity of PBMCs ranges from 80% to 90%, and the purity of CBMCs is greater than 90%; For umbilical cord blood CBMCs, successful cultivation has been achieved with umbilical cord blood cryopreserved in cord blood banks for up to 20 years.
The purity of CD3⁻CD56⁺ NK cells is > 98%, and the proportion of CD3⁺ cells is < 1%, which meets the requirements for autologous or allogeneic use; 50 mL of peripheral blood or umbilical cord blood can be used to expand NK cells to a quantity of 100 billion-level, with a large number of off-the-shelf NK products available; Sustainable culture can last for more than 28 days, with cell viability higher than 90%; Meets the requirements for NK cell new drug application.
NK cells derived from fresh blood samples are not affected by individual differences in NK cell purity among starting samples of peripheral blood and umbilical cord blood; With a stably maintained purity of >90%, they ensure the success rate of NK cell culture and compliance with quality release standards; Peripheral blood-derived NK cells are mainly used for autologous applications, and a certain amount of T cells (<10%) is permissible in such cases; For NK cell preparations in the big health sector, high cost-effectiveness is a core pursuit; One 50 mL fresh blood sample can be cultured once for use by clients in the big health sector.
Kit03 + Kit09: The Complete Solution for Cryopreservation, Recovery, and Reculture of NK Cell Seed Stocks.
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