3D StarPore®Max Large Health Spinner Flask System

1、500mL Spinner Flask, One StarSpan™ Mini Bioreactor

2、4 Flask Positions, with a Maximum System Volume of 2L at a Time

3、One 500mL spinner flask can hold a 400mL culture system, with an inoculation density of 2g/L (total 0.8g) and 4×10⁷ cells inoculated. After 20-fold expansion, 8×10⁸ cells can be harvested from a single flask in 4 days, which is equivalent to one 10-layer cell factory. One mini bioreactor can harvest 3.2×10⁹ cells at a time, equivalent to four 10-layer cell factories.

4、Reduce incubator occupancy rate, labor input, and cell processing workload.

3D StarPore®Max Large-Scale Production of Stem Cell Drugs

Expansion Protocol for 40 Billion Stem Cells in Secondary Tank

5L bioreactor: 1g/L (2L culture volume), inoculation of 1×10⁸ cells per 2g microcarriers, 20-fold expansion on day 4, harvest of 2×10⁹ cells

50L secondary bioreactor: 1g/L (40L culture volume), inoculation of 2×10⁹ cells, 20-fold expansion on day 4, harvest of 4×10¹⁰ cells

Expansion Protocol for 160 billion Stem Cells in Secondary Tank

10L bioreactor: 1g/L (8L reaction volume), inoculation of 4×10⁸ cells per 8g microcarriers, 20-fold expansion on day 4, harvest of 8×10⁹ cells 

200L secondary bioreactor: 1g/L (160L reaction volume), inoculation of 8×10⁹ cells, 20-fold expansion on day 4, harvest of 1.6×10¹¹ cells